OliLux’ solvatochromic-trehalose dyes were designed to label species within the actinobacteria phylum, including the pathogen that causes tuberculosis, Mycobacterium tuberculosis.
These dyes undergo fluorescence enhancement upon metabolic incorporation into the mycobacterial outer membrane as trehalose mycolates, performed by the acyl transferase Ag85 protein complex and its homologs (ref). DMN-Tre has been shown to also label Mycobacterium smegmatis, Corynebacterium glutamicum, and Mycobacterium marinum.
DMN (4-N,N-dimethylamino-1,8-naphthalimide)-trehalose. This product is supplied as a solution in ddH2O. DMN-Tre's optimal excitation/emission wavelength is 405 nm/525 nm respectively.
3-hydroxychromone (3HC)-trehalose. This product is supplied as a solution in DMSO. 3HC-3-Tre's optimal excitation/emission wavelength is 405 nm/525 nm, respectively. This dye is also visible under GFP conditions at 488/525 nm excitation/emission wavelengths.
For Research Use Only—Not for use in diagnostic procedures.
Store at –20°C for the short term and –80°C for the long term. Aliquot samples upon receipt as necessary to avoid repeated freeze thaw cycles.
Protocols and QC documentations available. Please contact us to inquire.
We are working with partners and medical institutions to clinically validate our diagnostic tests. If you are an investigator or care provider in tuberculosis, please drop us a note.